Recombinant Human IL-3 Protein, Animal Component-Free

Product Code: GFC0099

Catalog Number	Unit Size	Price
GFC0099-10 ug	10 ug	Online Inquiry
GFC0099-50 ug	50 ug	Online Inquiry
GFC0099-1 mg	1 mg	Online Inquiry

Description

Interleukin 3 (IL-3) is a pleiotropic factor produced primarily by activated T cells that can stimulate the proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils and macrophages.

Specification

Source

E. coli-derived human IL-3 protein, Ala20-Phe152, with and without an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.

Reactivity

Human

N-terminal Sequence

Ala20 and Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Molecular Mass (Predicted)

15 kDa

Accession

AAC08706.1

Biological Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.02-0.1 ng/mL.The specific activity of recombinant human IL-3 is >1.7 x 106 IU/mg, which is calibrated against the human IL-3 WHO International Standard (NIBSC code: 91/510).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.