Cytokines

Mouse IL-12

Product Code: GFC0078

Description

Interleukin 12 (IL-12) is a heterodimeric cytokine composed of p35 and p40 subunits. Upon binding, IL-12 initiates signaling via the JAK/STAT signaling pathway and stimulates NK, B, and T cells to produce IFN-γ. It also regulates cytokine synthesis, proliferation of T and NK cells, and stimulates differentiation of CD4+ and CD8+ T cells.

Specification

Source

HEK293

Symbols

CLMF, CTL maturation factor, Cytotoxic lymphocyte maturation factor, IL-12p70, Interleukin-12, Natural killer cells stimulator factor, NKSF

Accession

IL12A: P43431
IL12B: P43432

AA Sequence

IL12A:
RVIPVSGPARCLSQSRNLLKTTDDMVKTAREKLKHYSCTAEDIDHEDITRDQTSTLKTCLPLELHKNESCLATRETSSTTRGSCLPPQKTSLMMTLCLGSIYEDLKMYQTEFQAINAALQNHNHQQIILDKGMLVAIDELMQSLNHNGETLRQKPPVGEADPYRVKMKLCILLHAFSTRVVTINRVMGYLSSA
IL12B:
MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Molecular Mass

57.5 kDa

Purity

≥ 95%

Bioactivity

The specific activity as determined by the induced expression of CD25 on CD8+ mouse T cells is ≥ 5 x 10^4 units/mg (EC50 ≤ 20 ng/mL).

Storage

Store at -80°C. Avoid repeated freeze-thaw cycles.

For research or further manufacturing use only. Not for use in humans.