Recombinant Human FGF basic/FGF2/bFGF Protein, 146 aa, Animal Component-Free

Product Code: GFC0042

Catalog Number Unit Size Price
GFC0042-25 ug 25 ug Online Inquiry
GFC0042-1 mg 1 mg Online Inquiry

Description

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.

Specification

Source
E. coli-derived human FGF basic/FGF2/bFGF protein, Pro143-Ser288
Produced using non-animal reagents in an animal-free laboratory.
Reactivity
Human
N-terminal Sequence
Pro143 & Ala144
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Molecular Mass (Predicted)
16 kDa
Accession
NP_001997.5
Biological Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2 GMP is >8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).
Endotoxin
<0.01 EU per 1 μg of the protein by the LAL method.
Application
Bioactivity
For research or further manufacturing use only. Not for use in humans.

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