Recombinant Human HGF Protein, NS0-derived, GMP grade

Product Code: G-GFC0031

Catalog Number Unit Size Price
G-GFC0031-25 ug 25 ug Online Inquiry

Description

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.

Specification

Source
Mouse myeloma cell line, NS0-derived human HGF protein, Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Manufactured and tested under cGMP guidelines.
Reactivity
Human
N-terminal Sequence
Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32 Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu ( alpha -chain)Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn ( beta -chain)
Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions.
Molecular Mass (Predicted)
alpha chain: 53.7 kDa beta chain: 26 kDa
SDS-PAGE
71-85 kDa, non-reducing conditions56-65 kDa, and 30-40 kDa, reducing conditions
Accession
P14210.2
Biological Activity
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883. The ED50 for this effect is equal or less than 4.00 ng/mL.The specific activity of recombinant human HGF is >1.0 x 106 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
Endotoxin
<0.10 EU per 1 μg of the protein by the LAL method.
Application
Bioactivity
For research or further manufacturing use only. Not for use in humans.

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