Recombinant Human KGF/FGF-7 Protein, E. coli-derived, GMP grade

Product Code: G-GFC0023

Catalog Number	Unit Size	Price
G-GFC0023-50 ug	50 ug	Online Inquiry
G-GFC0023-1 mg	1 mg	Online Inquiry

Description

Keratinocyte growth factor (KGF), also known as fibroblast growth factor-7 (FGF-7), is part of the large FGF family, and alongside FGF-3, FGF-10, and FGF-22, is a member of the FGF-7 subfamily. KGF/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Recombinant human KGF/FGF-7 is an 18.9 kDa protein consisting of 163 amino acid residues.

Specification

Source

E. coli-derived human KGF/FGF-7 protein, Cys32-Thr194, with an N-terminal Met
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Met-(Cys)32-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met(Cys)32-Asn-Asp-Met-Thr-Pro-Glu-Gln-Met-Ala

Purity

>97%, by SDS-PAGE with silver staining, under reducing conditions.

Molecular Mass (Predicted)

19 kDa

SDS-PAGE

20 kDa, reducing conditions

Accession

P21781.1

Biological Activity

Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 6-60 ng/mL.The specific activity of recombinant human KGF/FGF-7 is >8.16 x 105 units/mg, which is calibrated against the human KGF/FGF-7 WHO Standard (NIBSC code: 03/150).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.