Recombinant Human FGF basic/FGF2 Protein, 146 aa, GMP grade

Product Code: G-GFC0021

Catalog Number	Unit Size	Price
G-GFC0021-25 ug	25 ug	Online Inquiry
G-GFC0021-1 mg	1 mg	Online Inquiry

Description

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation.

Specification

Source

E. coli-derived human FGF basic/FGF2/bFGF protein, Pro143-Ser288, with an N-terminal Ala
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Ala-Pro143-Ala-Leu-Pro-Glu-Asp-Gly-Gly-Ser

Purity

>97%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

16.5 kDa

SDS-PAGE

17 kDa, reducing conditions

Accession

P09038.3

Biological Activity

Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 0.1-0.6 ng/mL.The specific activity of Recombinant Human FGF basic/FGF2 GMP is >8.0 x 105 IU/mg, which is calibrated against the human FGF basic/FGF2 WHO International Standard (NIBSC code: 90/712).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.