Soluble Collagen Assay Kit

This kit allows for quantification of newly synthesized collagen levels in tissues and cultured cells and cell culture medium. The assay involves extraction of soluble collagen in acetic acid, followed by enzymatic degradation of collagen into glycine-rich oligopeptides, which are quantified using a fluorogenic reagent and developer solution that selectively react with the N-terminal glycine fragments to form a stable fluorescent complex.