Recombinant Human TGF-beta 1 Protein, GMP grade

Product Code: G-GFC0093

Catalog Number	Unit Size	Price
G-GFC0093-10 ug	10 ug	Online Inquiry

Description

Transforming growth factor beta 1 (TGF-β1) is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. Recombinant human TGF-β1 is used for the cultivation of embryonic stem cells and induced pluripotent stem cells, but can also promote Th17 and Treg differentiation of T cells.

Specification

Source

Chinese Hamster Ovary cell line, CHO-derived human TGF-beta 1 protein, Ala279-Ser390
Manufactured and tested under cGMP guidelines.

Reactivity

Human

N-terminal Sequence

Ala-Leu-Asp-Thr-Asn-Tyr-(Cys)-Phe-Ser-Ser

Purity

>97%, by SDS-PAGE with silver staining.

Molecular Mass (Predicted)

12.8 kDa (monomer)

SDS-PAGE

12 kDa, reducing conditions24 kDa, non-reducing conditions

Accession

P01137.2

Biological Activity

Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.04-0.2 ng/mL.The specific activity of recombinant human TGF-beta 1 GMP is >1.0 x 107 units/mg, which is calibrated against the human TGF‑ beta 1 Standard (NIBSC code: 89/514).

Endotoxin

<0.10 EU per 1 μg of the protein by the LAL method.

Application

Bioactivity

For research or further manufacturing use only. Not for use in humans.