Recombinant Human EGF Protein, GMP grade

Product Code: G-GFC0014

Catalog Number Unit Size Price
G-GFC0014-200 ug 200 ug Online Inquiry
G-GFC0014-1 mg 1 mg Online Inquiry

Description

Epidermal growth factor (EGF) is a member of the EGF-family and plays an important role in the regulation of cell growth, proliferation, and differentiation through binding to its receptor EGFR. Recombinant human EGF is optimized for use in cell culture, differentiation studies, and functional assays.

Specification

Source
E. coli-derived human EGF protein, Asn971-Arg1023, with an N-terminal Met
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.
Reactivity
Human
N-terminal Sequence
Met-Asn971-Ser-Asp-Ser-Glu-(Cys)-Pro-Leu-Ser
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. The result of the major peak from mass spectrometry analysis is 6347 Da, which corresponds to the calculated molecular mass of 6353 Da.
Molecular Mass (Predicted)
6 kDa
SDS-PAGE
6 kDa, reducing conditions
Accession
P01133.2
Biological Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20‑100 pg/mL.The specific activity of Recombinant Human EGF is >8.0 x 105 IU/mg, which is calibrated against the human EGF WHO International Standard (NIBSC code: 91/530).
Endotoxin
<0.10 EU per 1 μg of the protein by the LAL method.
Application
Bioactivity
For research or further manufacturing use only. Not for use in humans.

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